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Aging of mesenchymal stem cell in vitro

Mandana Mohyeddin Bonab, Kamran Alimoghaddam, Fatemeh Talebian, Seyed H. Ghaffari, Ardeshir Ghavamzadeh, Behrouz Nikbin

BMC Cell Biology · 2006 · ▲ 897 citations

Telomere attrition Cellular senescence Stem-cell exhaustion Gene therapy Stem-cell therapy Cell culture / in vitro Human In vitro

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Abstract

BACKGROUND: A hot new topic in medical treatment is the use of mesenchymal stem cells (MSC) in therapy. The low frequency of this subpopulation of stem cells in bone marrow (BM) necessitates their in vitro expansion prior to clinical use. We evaluated the effect of long term culture on the senescence of these cells. RESULTS: The mean long term culture was 118 days and the mean passage number was 9. The average number of PD decreased from 7.7 to 1.2 in the 10th passage. The mean telomere length decreased from 9.19 Kbp to 8.7 kbp in the 9th passage. Differentiation potential dropped from the 6th passage on. The culture's morphological abnormalities were typical of the Hayflick model of cellular aging. CONCLUSION: We believe that MSC enter senescence almost undetectably from the moment of in vitro culturing. Simultaneously these cells are losing their stem cell characteristics. Therefore, it is much better to consider them for cell and gene therapy early on.

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Provenance

Source: OpenAlex
DOI: 10.1186/1471-2121-7-14
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Fetched: 2026-06-19 MST

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APA

Bonab, M.M., Alimoghaddam, K., Talebian, F., Ghaffari, S.H., Ghavamzadeh, A., & Nikbin, B. (2006). Aging of mesenchymal stem cell in vitro. <em>BMC Cell Biology</em>. https://doi.org/10.1186/1471-2121-7-14

Vancouver

Bonab MM, Alimoghaddam K, Talebian F, Ghaffari SH, Ghavamzadeh A, Nikbin B. Aging of mesenchymal stem cell in vitro. BMC Cell Biology. 2006. doi:10.1186/1471-2121-7-14.

BibTeX

@article{mandana2006Agingo, title = {Aging of mesenchymal stem cell in vitro}, author = {Mandana Mohyeddin Bonab and Kamran Alimoghaddam and Fatemeh Talebian and Seyed H. Ghaffari and Ardeshir Ghavamzadeh and Behrouz Nikbin}, journal = {BMC Cell Biology}, year = {2006}, doi = {10.1186/1471-2121-7-14}, }