Additional file 4 of Phosphatidylinositol transfer protein-1 integrates insulin/IGF-1 and TOR signaling to negatively regulate lifespan and healthspan in Caenorhabditis elegans

Supplementary material 4. Supplementary Fig. 4. The role of pitp-1 in IIS-mediated lifespan regulation. (A) Knockdown of pitp-1 did not promote DAF-16 nuclear translocation. TJ356[daf-16p::daf-16a/b::GFP + rol-6(su1006)] was used as a DAF-16 reporter strain. Red arrows indicated DAF-16::GFP translocated into the nucleus and forms GFP puncta by daf-2(RNAi) as the positive control. (B, C) Knockdown of pitp-1 did not increase sod-3 expression. CF1553[sod-3p::GFP + rol-6(su1006)] was used as a sod-3 reporter strain. (D) Whole-genome microarray data from C. elegans [45] revealed pitp-1 expression was significantly reduced in daf-2(e1370). (E) PITP-1 overexpression partially blocked the longevity effect by age-1(RNAi) knockdown. Survival curves are representative of three independent experiments. Data are presented as mean ± SD (n = 3 biological replicates) for quantitative analyses. Statistical significance was determined by log-rank test for lifespan assays, ANOVA for multiple comparisons, and unpaired Student’s t-test where applicable.