Additional file 3 of Phosphatidylinositol transfer protein-1 integrates insulin/IGF-1 and TOR signaling to negatively regulate lifespan and healthspan in Caenorhabditis elegans

Supplementary material 3. Supplementary Fig. 3. pitp-1 negatively regulates lifespan by modulating TOR signaling. (A–B) RNAi knockdown of pitp-1 did not further prolong the extended lifespan in two dgk-5 mutants. (C, D) The elevated p-S6K levels in PITP-1 overexpression strains were reverted by genetic inhibition of TOR signaling (let-363, raga-1). (E, F) The reduced p-S6K levels in two pitp-1 mutants were reverted by PITP-1 overexpression. (G, H) Genetic or pharmacological inhibition of TOR did not further enhance the extended lifespan in pitp-1(tm1500) mutant. (I) Knockdown of pitp-1 did not further prolong the enhanced lifespan in rsks-1 mutants. (J) Genetic knockdown of TOR by let-363(RNAi) rescued the motility decline caused by PITP-1 overexpression. (K) Schematic diagram of TOR upstream regulators RAG, RHEB, AMPK. (L) Knockdown of pitp-1 extended lifespan in aak-2(gt33). (M, N) sesn-1 mutation blocked the longevity effect of pitp-1(RNAi) knockdown. Survival curves are representative of at least two independent experiments (n = 2–3 depending on the strain). Data are presented as mean ± SD (n = 3—4 independent experiments) for quantitative analyses. Statistical significance was determined by log-rank test for lifespan assays, ANOVA for multiple comparisons.