A comparative study of anti-aging properties and mechanism: resveratrol and caloric restriction

// Juan Li 1 , Chun-Xia Zhang 1 , Yi-Mei Liu 1 , Ke-Li Chen 1 and Gang Chen 1, 2 1 Key Laboratory of Ministry of Education on Traditional Chinese Medicine Resource and Compound Prescription, Hubei University of Chinese Medicine, Wuhan 430065, Hubei, China 2 Hubei College of Chinese Medicine, Jingzhou 434020, Hubei, China Correspondence to: Gang Chen, email: [email protected] Keywords: resveratrol, caloric restriction(definition), SIRT1, aging Received: June 12, 2017      Accepted: July 25, 2017      Published: August 09, 2017 ABSTRACT Resveratrol and caloric restriction (CR) are the powerful therapeutic options for anti-aging. Here, their comparative effect on longevity-associated gene silencing information regulator (SIRT1) were evaluated in vitro and in vivo . IMR-90 cells treated with 2,2’-azobis (2-amidinopropane) dihydrochloride (AAPH) were applied to establish a cellular senescence(definition) model, and rats treated with D-galactose (D-gal) were used as an aging animal model. Resveratrol and CR exhibited similar anti-aging activities, evidenced by inhibiting senescence and apoptosis, and restoring cognitive impairment and oxidative damage. Moreover, they could up-regulate telomerase (TE) activity, increase expressions of SIRT1, forkhead box 3a (Foxo3a), active regulator of SIRT1 (AROS) and Hu antigen R (HuR ), but decrease p53 and deleted in breast cancer 1 (DBC1) levels. However, 10 μM resveratrol in vitro and the high dose group in vivo showed relatively stronger activities of anti-aging and stimulating SIRT1 level than CR. In conclusion, resveratrol and CR showed similar anti-aging activities on SIRT1 signaling, implicating the potential of resveratrol as a CR mimetic.