The effect of resveratrol on Caenorhabditis elegans lifespan and its molecular mechanism

Objective:To study the extending longevity effect and anti-senescence(definition) molecular mechanism of resveratrol. Method:The lifespan assay was carried out on model organism Caenorhabditis elegans(C. elegans)which dieted resveratrol with final concentration 50mg/L and 100mg/L under normal culture conditions(25℃)and under heat stress(35℃),respectively. Semi-quantitative RT-PCR was used to analyze the expression of aging-associated genes such as daf-16,sod-3 and hsp-16. 2. Result:Under the two kinds of culture conditions the C. elegans fed resveratrol was shown to extend lifespan compared to the control without resveratrol uptake. Furthermore,the resveratrol with final concentration 100mg/L had more obvious longevity-extending effect. The expression of aging-associated genes was up-regulated. Conclusion:The results suggested that resveratrol significantly extended the lifespan of C. elegans through up-regulating some genes’ expression such as daf-16,sod-3 and hsp-16. 2 which would provide important reference for antiaging research of resveratrol.