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Technical challenges of working with extracellular vesicles

Marcel I. Ramirez, María Galli de Amorim, Catarina Gadelha, Ivana Milic, Joshua A Welsh, Vanessa M. Freitas, Muhammad Nawaz, Naveed Akbar, Yvonne Couch, Laura Makin, Fiona G. M. Cooke, André L. Vettore, Patricia X. Batista, Roberta Cordeiro Freezor, Julia Alejandra Pezuk

Nanoscale · 2017 · ▲ 520 citations

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Abstract

Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.

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Provenance

Source: OpenAlex
DOI: 10.1039/c7nr08360b
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Fetched: 2026-07-28 MST

Cite this

APA

Ramirez, M.I., Amorim, M.G.D., Gadelha, C., Milic, I., Welsh, J.A., Freitas, V.M., Nawaz, M., Akbar, N., Couch, Y., Makin, L., Cooke, F.G.M., Vettore, A.L., Batista, P.X., Freezor, R.C., Pezuk, J.A., Rosa-Fernandes, L., Carreira, A.C.O., Devitt, A., Jacobs, L.A., & Silva, I.T.D. (2017). Technical challenges of working with extracellular vesicles. <em>Nanoscale</em>. https://doi.org/10.1039/c7nr08360b

Vancouver

Ramirez MI, Amorim MGD, Gadelha C, Milic I, Welsh JA, Freitas VM, et al. Technical challenges of working with extracellular vesicles. Nanoscale. 2017. doi:10.1039/c7nr08360b.

BibTeX

@article{marcel2017Techni, title = {Technical challenges of working with extracellular vesicles}, author = {Marcel I. Ramirez and María Galli de Amorim and Catarina Gadelha and Ivana Milic and Joshua A Welsh and Vanessa M. Freitas and Muhammad Nawaz and Naveed Akbar and Yvonne Couch and Laura Makin and Fiona G. M. Cooke and André L. Vettore and Patricia X. Batista and Roberta Cordeiro Freezor and Julia Alejandra Pezuk and Lívia Rosa-Fernandes and Ana Cláudia Oliveira Carreira and Andrew Devitt and Laura A. Jacobs and Israel Tojal da Silva and Gillian Coakley and Diana Noronha Nunes and Dave Carter and Giuseppe Palmisano and Emmanuel Dias‐Neto}, journal = {Nanoscale}, year = {2017}, doi = {10.1039/c7nr08360b}, }