Open access · OA via OpenAlex

Methodological Guidelines to Study Extracellular Vesicles

Frank A. W. Coumans, Alain Brisson, Edit I. Buzás, Françoise Dignat‐George, Esther E.E. Drees, Samir EL Andaloussi, Costanza Emanueli, Aleksandra Gąsecka, An Hendrix, Andrew F. Hill, Romaric Lacroix, Yi Lee, Ton G. van Leeuwen, Nigel Mackman, Imre Mäger

Circulation Research · 2017 · ▲ 1,040 citations

Dysbiosis Human Review

＋ Sign in to save

Abstract

Owing to the relationship between extracellular vesicles (EVs) and physiological and pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes for novel diagnostic and translational discoveries. This review provides an expert-based update of recent advances in the methods to study EVs and summarizes currently accepted considerations and recommendations from sample collection to isolation, detection, and characterization of EVs. Common misconceptions and methodological pitfalls are highlighted. Although EVs are found in all body fluids, in this review, we will focus on EVs from human blood, not only our most complex but also the most interesting body fluid for cardiovascular research.

◌ CITATION ONLY
Full text is not openly licensed for redistribution here. Read it at the source:

Read at source →

Provenance

Source: OpenAlex
DOI: 10.1161/circresaha.117.309417
Canonical: link ↗
Fetched: 2026-06-08 MST

Cite this

APA

Coumans, F.A.W., Brisson, A., Buzás, E.I., Dignat‐George, F., Drees, E.E., Andaloussi, S.E., Emanueli, C., Gąsecka, A., Hendrix, A., Hill, A.F., Lacroix, R., Lee, Y., Leeuwen, T.G.V., Mackman, N., Mäger, I., Nolan, J.P., Pol, E.V.D., Pegtel, D.M., Sahoo, S., & Siljander, P. (2017). Methodological Guidelines to Study Extracellular Vesicles. <em>Circulation Research</em>. https://doi.org/10.1161/circresaha.117.309417

Vancouver

Coumans FAW, Brisson A, Buzás EI, Dignat‐George F, Drees EE, Andaloussi SE, et al. Methodological Guidelines to Study Extracellular Vesicles. Circulation Research. 2017. doi:10.1161/circresaha.117.309417.

BibTeX

@article{frank2017Method, title = {Methodological Guidelines to Study Extracellular Vesicles}, author = {Frank A. W. Coumans and Alain Brisson and Edit I. Buzás and Françoise Dignat‐George and Esther E.E. Drees and Samir EL Andaloussi and Costanza Emanueli and Aleksandra Gąsecka and An Hendrix and Andrew F. Hill and Romaric Lacroix and Yi Lee and Ton G. van Leeuwen and Nigel Mackman and Imre Mäger and John P. Nolan and Edwin van der Pol and D. Michiel Pegtel and Susmita Sahoo and Pia Siljander and Guus Sturk and Olivier De Wever and Rienk Nieuwland}, journal = {Circulation Research}, year = {2017}, doi = {10.1161/circresaha.117.309417}, }