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Sirtuin 1 regulation of developmental genes during differentiation of stem cells

Vincenzo Calvanese, Ester Lara, Beatriz Suárez-Álvarez, Raed Abu Dawud, Mercedes Vázquez–Chantada, Maria Luz Martínez‐Chantar, Nieves Embade, Pilar López‐Nieva, Angélica Horrillo, Abdelkrim Hmadcha, Bernat Soria, Daniela Piazzolla, Daniel Herranz, Manuel Serrano, José María Mato

Proceedings of the National Academy of Sciences · 2010 · ▲ 164 citations

Epigenetic alterations Deregulated nutrient-sensing Stem-cell exhaustion Human

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Abstract

The longevity-promoting NAD+-dependent class III histone deacetylase Sirtuin 1 (SIRT1) is involved in stem cell function by controlling cell fate decision and/or by regulating the p53-dependent expression of NANOG. We show that SIRT1 is down-regulated precisely during human embryonic stem cell differentiation at both mRNA and protein levels and that the decrease in Sirt1 mRNA is mediated by a molecular pathway that involves the RNA-binding protein HuR and the arginine methyltransferase coactivator-associated arginine methyltransferase 1 (CARM1). SIRT1 down-regulation leads to reactivation of key developmental genes such as the neuroretinal morphogenesis effectors DLL4, TBX3, and PAX6, which are epigenetically repressed by this histone deacetylase in pluripotent human embryonic stem cells. Our results indicate that SIRT1 is regulated during stem cell differentiation in the context of a yet-unknown epigenetic pathway that controls specific developmental genes in embryonic stem cells.

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Provenance

Source: OpenAlex
DOI: 10.1073/pnas.1001399107
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Fetched: 2026-10-22 MST

Cite this

APA

Calvanese, V., Lara, E., Suárez-Álvarez, B., Dawud, R.A., Vázquez–Chantada, M., Martínez‐Chantar, M.L., Embade, N., López‐Nieva, P., Horrillo, A., Hmadcha, A., Soria, B., Piazzolla, D., Herranz, D., Serrano, M., Mato, J.M., Andrews, P.W., López‐Larrea, C., Esteller, M., & Fraga, M.F. (2010). Sirtuin 1 regulation of developmental genes during differentiation of stem cells. <em>Proceedings of the National Academy of Sciences</em>. https://doi.org/10.1073/pnas.1001399107

Vancouver

Calvanese V, Lara E, Suárez-Álvarez B, Dawud RA, Vázquez–Chantada M, Martínez‐Chantar ML, et al. Sirtuin 1 regulation of developmental genes during differentiation of stem cells. Proceedings of the National Academy of Sciences. 2010. doi:10.1073/pnas.1001399107.

BibTeX

@article{vincenzo2010Sirtui, title = {Sirtuin 1 regulation of developmental genes during differentiation of stem cells}, author = {Vincenzo Calvanese and Ester Lara and Beatriz Suárez-Álvarez and Raed Abu Dawud and Mercedes Vázquez–Chantada and Maria Luz Martínez‐Chantar and Nieves Embade and Pilar López‐Nieva and Angélica Horrillo and Abdelkrim Hmadcha and Bernat Soria and Daniela Piazzolla and Daniel Herranz and Manuel Serrano and José María Mato and Peter W. Andrews and Carlos López‐Larrea and Manel Esteller and Mario F. Fraga}, journal = {Proceedings of the National Academy of Sciences}, year = {2010}, doi = {10.1073/pnas.1001399107}, }