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Protocol for whole-brain immunostaining of the turquoise killifish after tissue clearing

STAR Protocols · 2021 · ▲ 2 citations

Telomere attrition Killifish

Abstract

Distribution of cells and proteins in whole organs, such as the brain, can provide another level of insight into molecular and cellular functions. Here, we describe a whole-brain immunostaining method using the turquoise killifish, an emerging model for aging research. We optimized a protocol for tissue clearing and whole-brain immunostaining to the turquoise killifish brain. This protocol provides a comprehensive procedure from brain dissection to whole-brain imaging and image processing. For complete details on the use and execution of this protocol, please refer to Eunjeong Do (2020) and Lee et al. (2021).

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Provenance

Source: OpenAlex
DOI: 10.1016/j.xpro.2021.100564
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Fetched: 2026-08-04 MST

Cite this

APA

Lee, S., & Kim, Y. (2021). Protocol for whole-brain immunostaining of the turquoise killifish after tissue clearing. <em>STAR Protocols</em>. https://doi.org/10.1016/j.xpro.2021.100564

Vancouver

Lee S, Kim Y. Protocol for whole-brain immunostaining of the turquoise killifish after tissue clearing. STAR Protocols. 2021. doi:10.1016/j.xpro.2021.100564.

BibTeX

@article{seongsin2021Protoc, title = {Protocol for whole-brain immunostaining of the turquoise killifish after tissue clearing}, author = {Seongsin Lee and Yumi Kim}, journal = {STAR Protocols}, year = {2021}, doi = {10.1016/j.xpro.2021.100564}, }