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Protocol for systematic screening of histone H3/H4 mutants to identify histone residues critical for BCAA-dependent replicative lifespan.

STAR protocols · 2025

Epigenetic alterations Yeast

Abstract

Here, we present a protocol for high-throughput replicative lifespan (RLS) screening in Saccharomyces cerevisiae using micromanipulation-based aging assays with growth phenotyping. We describe steps for preparing yeast strains, isolating virgin cells, and monitoring divisions to calculate lifespan changes in histone H3/H4 mutants. We also describe sulfometuron methyl (SM) spotting assay to evaluate growth phenotypes linked to branched-chain amino acid (BCAA) metabolism, enabling identification of residues critical for lifespan regulation. For complete details on the use and execution of this protocol, please refer to Park et al.<sup>1</sup>.

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Provenance

Source: Europe PMC
DOI: 10.1016/j.xpro.2025.104243
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Fetched: 2026-07-01 MST

Cite this

APA

S, P., Y, L., & SH., A. (2025). Protocol for systematic screening of histone H3/H4 mutants to identify histone residues critical for BCAA-dependent replicative lifespan. <em>STAR protocols</em>. https://doi.org/10.1016/j.xpro.2025.104243

Vancouver

S P, Y L, SH. A. Protocol for systematic screening of histone H3/H4 mutants to identify histone residues critical for BCAA-dependent replicative lifespan. STAR protocols. 2025. doi:10.1016/j.xpro.2025.104243.

BibTeX

@article{park2025Protoc, title = {Protocol for systematic screening of histone H3/H4 mutants to identify histone residues critical for BCAA-dependent replicative lifespan.}, author = {Park S and Liu Y and Ahn SH.}, journal = {STAR protocols}, year = {2025}, doi = {10.1016/j.xpro.2025.104243}, }