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Protocol for systematic screening of histone H3/H4 mutants to identify histone residues critical for BCAA-dependent replicative lifespan.
STAR protocols · 2025
Abstract
Here, we present a protocol for high-throughput replicative lifespan (RLS) screening in Saccharomyces cerevisiae using micromanipulation-based aging assays with growth phenotyping. We describe steps for preparing yeast strains, isolating virgin cells, and monitoring divisions to calculate lifespan changes in histone H3/H4 mutants. We also describe sulfometuron methyl (SM) spotting assay to evaluate growth phenotypes linked to branched-chain amino acid (BCAA) metabolism, enabling identification of residues critical for lifespan regulation. For complete details on the use and execution of this protocol, please refer to Park et al.<sup>1</sup>.
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Provenance
- Source
- Europe PMC
- DOI
- 10.1016/j.xpro.2025.104243
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- link ↗
- Fetched
- 2026-07-01 MST
Cite this
APA
S, P., Y, L., & SH., A. (2025). Protocol for systematic screening of histone H3/H4 mutants to identify histone residues critical for BCAA-dependent replicative lifespan. <em>STAR protocols</em>. https://doi.org/10.1016/j.xpro.2025.104243
Vancouver
S P, Y L, SH. A. Protocol for systematic screening of histone H3/H4 mutants to identify histone residues critical for BCAA-dependent replicative lifespan. STAR protocols. 2025. doi:10.1016/j.xpro.2025.104243.
BibTeX
@article{park2025Protoc,
title = {Protocol for systematic screening of histone H3/H4 mutants to identify histone residues critical for BCAA-dependent replicative lifespan.},
author = {Park S and Liu Y and Ahn SH.},
journal = {STAR protocols},
year = {2025},
doi = {10.1016/j.xpro.2025.104243},
}
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