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A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates

Andrey A. Petropavlovskiy, Michael G. Tauro, Patrick Lajoie, Martin L. Duennwald

STAR Protocols · 2020 · ▲ 89 citations

Stem-cell exhaustion Yeast

Abstract

We present a detailed protocol that describes the evaluation of the growth and survival of yeast cells by quantitatively analyzing spotting assays. This simple method reproducibly detects and quantifies subtle differences in growth by measuring the density of cells within a single spot of defined size on an image of a spotting assay. Our protocol is tailored specifically for low-throughput applications, can be easily adapted for specific experimental conditions, and is accessible to yeast experts and non-experts alike. For an example of the execution of this protocol, please refer to DiGregorio et al. (Di Gregorio et al., 2020).

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Provenance

Source: OpenAlex
DOI: 10.1016/j.xpro.2020.100182
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Fetched: 2026-09-09 MST

Cite this

APA

Petropavlovskiy, A.A., Tauro, M.G., Lajoie, P., & Duennwald, M.L. (2020). A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates. <em>STAR Protocols</em>. https://doi.org/10.1016/j.xpro.2020.100182

Vancouver

Petropavlovskiy AA, Tauro MG, Lajoie P, Duennwald ML. A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates. STAR Protocols. 2020. doi:10.1016/j.xpro.2020.100182.

BibTeX

@article{andrey2020AQuant, title = {A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates}, author = {Andrey A. Petropavlovskiy and Michael G. Tauro and Patrick Lajoie and Martin L. Duennwald}, journal = {STAR Protocols}, year = {2020}, doi = {10.1016/j.xpro.2020.100182}, }