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A novel assay for replicative lifespan in

Stefanie Jarolim, Jonathan I. Millen, Gino Heeren, Peter Laun, David S. Goldfarb, Michael Breitenbach

FEMS Yeast Research · 2004 · ▲ 104 citations

Stem-cell exhaustion Yeast

Abstract

The replicative lifespan of Saccharomyces cerevisiae is determined by both genetic and environmental factors. Many of the same factors determine the lifespan of metazoan animals. The lack of fast and reliable lifespan assays has limited the pace of yeast aging research. In this study we describe a novel strategy for assaying replicative lifespan in yeast, and apply it in a screening of mutants that are resistant to pro-oxidants. The assay reproduces the lifespan-shortening effects of deleting SIR2 and of growth in the presence of paraquat, a pro-oxidant. The lifespan-increasing activity of resveratrol is also reproduced. Compared to current assays, this new strategy promises to significantly increase the possible number of replicative-lifespan determinations.

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Provenance

Source: OpenAlex
DOI: 10.1016/j.femsyr.2004.06.015
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Fetched: 2026-06-15 MST

Cite this

APA

Jarolim, S., Millen, J.I., Heeren, G., Laun, P., Goldfarb, D.S., & Breitenbach, M. (2004). A novel assay for replicative lifespan in. <em>FEMS Yeast Research</em>. https://doi.org/10.1016/j.femsyr.2004.06.015

Vancouver

Jarolim S, Millen JI, Heeren G, Laun P, Goldfarb DS, Breitenbach M. A novel assay for replicative lifespan in. FEMS Yeast Research. 2004. doi:10.1016/j.femsyr.2004.06.015.

BibTeX

@article{stefanie2004Anovel, title = {A novel assay for replicative lifespan in}, author = {Stefanie Jarolim and Jonathan I. Millen and Gino Heeren and Peter Laun and David S. Goldfarb and Michael Breitenbach}, journal = {FEMS Yeast Research}, year = {2004}, doi = {10.1016/j.femsyr.2004.06.015}, }