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Naked Mole Rat Cells Have a Stable Epigenome that Resists iPSC Reprogramming

Li Tan, Zhonghe Ke, Gregory Tombline, Nicholas Macoretta, Kevin Hayes, Xiao Tian, Ruitu Lv, Julia Ablaeva, Michael Gilbert, Natarajan V. Bhanu, Zuo‐Fei Yuan, Benjamin A. Garcia, Yujiang Geno Shi, Yang Shi, Andrei Seluanov

Stem Cell Reports · 2017 · ▲ 88 citations

Epigenetic alterations Partial reprogramming (OSK) Cell culture / in vitro Rat Mouse Naked mole-rat

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Abstract

Naked mole rat (NMR) is a valuable model for aging and cancer research due to its exceptional longevity and cancer resistance. We observed that the reprogramming efficiency of NMR fibroblasts in response to OSKM was drastically lower than that of mouse fibroblasts. Expression of SV40 LargeT antigen (LT) dramatically improved reprogramming of NMR fibroblasts. Inactivation of Rb alone, but not p53, was sufficient to improve reprogramming efficiency, suggesting that NMR chromatin may be refractory to reprogramming. Analysis of the global histone landscape revealed that NMR had higher levels of repressive H3K27 methylation marks and lower levels of activating H3K27 acetylation marks than mouse. ATAC-seq revealed that in NMR, promoters of reprogramming genes were more closed than mouse promoters, while expression of LT led to massive opening of the NMR promoters. These results suggest that NMR displays a more stable epigenome that resists de-differentiation, contributing to the cancer resistance and longevity of this species.

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Provenance

Source: OpenAlex
DOI: 10.1016/j.stemcr.2017.10.001
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Fetched: 2026-08-04 MST

Cite this

APA

Tan, L., Ke, Z., Tombline, G., Macoretta, N., Hayes, K., Tian, X., Lv, R., Ablaeva, J., Gilbert, M., Bhanu, N.V., Yuan, Z., Garcia, B.A., Shi, Y.G., Shi, Y., Seluanov, A., & Gorbunova, V. (2017). Naked Mole Rat Cells Have a Stable Epigenome that Resists iPSC Reprogramming. <em>Stem Cell Reports</em>. https://doi.org/10.1016/j.stemcr.2017.10.001

Vancouver

Tan L, Ke Z, Tombline G, Macoretta N, Hayes K, Tian X, et al. Naked Mole Rat Cells Have a Stable Epigenome that Resists iPSC Reprogramming. Stem Cell Reports. 2017. doi:10.1016/j.stemcr.2017.10.001.

BibTeX

@article{li2017NakedM, title = {Naked Mole Rat Cells Have a Stable Epigenome that Resists iPSC Reprogramming}, author = {Li Tan and Zhonghe Ke and Gregory Tombline and Nicholas Macoretta and Kevin Hayes and Xiao Tian and Ruitu Lv and Julia Ablaeva and Michael Gilbert and Natarajan V. Bhanu and Zuo‐Fei Yuan and Benjamin A. Garcia and Yujiang Geno Shi and Yang Shi and Andrei Seluanov and Vera Gorbunova}, journal = {Stem Cell Reports}, year = {2017}, doi = {10.1016/j.stemcr.2017.10.001}, }