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In Vitro Fertilization of the African Turquoise Killifish<i>Nothobranchius furzeri</i>

Luca Dolfi, Tsz Kin Suen, Danel Medelbekova, Roberto Ripa, Orsolya Symmons, Adam Antebi

Cold Spring Harbor Protocols · 2023 · ▲ 1 citations

Telomere attrition Killifish Cell culture / in vitro In vitro
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Abstract

The ability to perform in vitro fertilization, together with sperm cryopreservation, greatly facilitates the long-term laboratory maintenance of wild-type and transgenic model organisms and helps prevent genetic drift. It is also useful in cases where reproduction may be compromised. In this protocol, we present a method for in vitro fertilization of the African Turquoise killifish Nothobranchius furzeri that is compatible with the use of fresh or cryopreserved sperm.

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Provenance

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OpenAlex
DOI
10.1101/pdb.prot107886
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2026-07-07 MST

Cite this

APA
Dolfi, L., Suen, T.K., Medelbekova, D., Ripa, R., Symmons, O., &amp; Antebi, A. (2023). In Vitro Fertilization of the African Turquoise Killifish<i>Nothobranchius furzeri</i>. <em>Cold Spring Harbor Protocols</em>. https://doi.org/10.1101/pdb.prot107886
Vancouver
Dolfi L, Suen TK, Medelbekova D, Ripa R, Symmons O, Antebi A. In Vitro Fertilization of the African Turquoise Killifish<i>Nothobranchius furzeri</i>. Cold Spring Harbor Protocols. 2023. doi:10.1101/pdb.prot107886.
BibTeX
@article{luca2023InVitr, title = {In Vitro Fertilization of the African Turquoise Killifish<i>Nothobranchius furzeri</i>}, author = {Luca Dolfi and Tsz Kin Suen and Danel Medelbekova and Roberto Ripa and Orsolya Symmons and Adam Antebi}, journal = {Cold Spring Harbor Protocols}, year = {2023}, doi = {10.1101/pdb.prot107886}, }

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