Effect of Over-expression of CG9940 Gene on Cardiac Function,Exercise Ability and Lifespan in Old Drosophila Melanogaster

Objective: This study explored the effect of over-expression of CG9940 on anti-aging cardiac function,exercise ability and lifespan in drosophila melanogaster by using Gal4 /UAS system. Methods: The control group drosophila melanogasters are the filial generation of W1118female drosophila melanogasters crossing with arm-Gal4males,and the over-expression group ones are the filial generation of y[1]w[67c23]P{ y[+ m8] = Mae-UAS. 6. 11}CG9940[GG01267]females crossing with arm-Gal4 males. One thousand and two hundreds virgin drosophila melanogasters in each group were tested when they were one-week,three-week,5- week and seventh-week old. The mRNA expression of CG9940 gene was detected by real-time PCR. The diastolic dysfunction index and arrhythmia index were detected by M-mode echocardiography,and climbing index and lifespan were calculated. Results: The mRNA expression of CG9940 gene was higher in over-expression group than in control group( P 0. 01) and the over-expression rate is 144. 09%. Diastolic dysfunction index of seven-week old drosophila melanogasters in control group is higher than that in over-expression group( P 0. 01),and it increased significantly from five-week old to seven-week old in control group( P 0. 01). Arrhythmia index of one-week,five-week and seven-week old ones in control group is higher than that in over-expression group respectively( P 0. 05),and it increased significantly from three-week old to five-week old in control group( P 0. 05). The climbing index of one-week old and three-week old drosophila melanogaster in control group is lower than that in over-expression group( P 0. 01).The average lifespan in over-expression group is 20. 4% longer than that in control group( P 0. 01); the oldest one in over-expression group is 77 days old while it is 66 days old in control group. Conclusion: Over-expression of CG9940 gene can protect old drosophila melanogasters' cardiac function,maintain and improve their exercise ability,and prolong their lifespan,which indicates that CG9940 gene is closely related to aging of cardiac function,exercise ability and lifespan.