Stability of natural polyphenol fisetin in eye drops Stability of fisetin in eye drops

Abstract Fisetin is a polyphenolic compound with anti-inflammatory and antioxidant properties. Inflammation and reactive oxygen species play a major role in the pathophysiology of the dry eye syndrome (DES). Patients with DES undergo symptomatic treatment using eye drops known as artificial tears. Addition of fisetin into the eye drops could result in a better recovery of the eye surface. This experimental study examines the stability of fisetin in selected eye drops (Arufil, Hypromelóza-P, Ocutein, Refresh). Absorption spectra of fisetin were measured in selected eye drops, dimethylsulphoxide (DMSO), deionized water and normal saline solution (NSS) during a period of four weeks. The fisetin absorption maximum was placed at 350 – 390 nm depending on the solvent. Good stability of fisetin solutions were observed in DMSO and deionized water. The highest stability of fisetin in selected eye drops was observed in Hypromelóza-P. Irreversible fisetin structural changes were detected in Arufil, Ocutein, Refresh and NSS. For further clinical evaluation, fisetin solution in Hypromelóza-P could be examined.