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Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors

Warren Young, Timothy A. Steele, Robert A. Bray, John Hudson, Julie A. Floyd, Kristina C. Hawkins, Karen Thomas, Troy Austin, Chris Edwards, Jeremy Cuzzourt, Mary Duenzl, Paul A. Lucas, Asa C. Black

The Anatomical Record · 2001 · ▲ 541 citations

Stem-cell exhaustion Stem-cell therapy Cell culture / in vitro Human

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Abstract

This study details the profile of 13 cell surface cluster differentiation markers on human reserve stem cells derived from connective tissues. Stem cells were isolated from the connective tissues of dermis and skeletal muscle derived from fetal, mature, and geriatric humans. An insulin/dexamethasone phenotypic bioassay was used to determine the identity of the stem cells from each population. All populations contained lineage-committed myogenic, adipogenic, chondrogenic, and osteogenic progenitor stem cells as well as lineage-uncommitted pluripotent stem cells capable of forming muscle, adipocytes, cartilage, bone, fibroblasts, and endothelial cells. Flow cytometric analysis of adult stem cell populations revealed positive staining for CD34 and CD90 and negative staining for CD3, CD4, CD8, CD11c, CD33, CD36, CD38, CD45, CD117, Glycophorin-A, and HLA DR-II.

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Provenance

Source: OpenAlex
DOI: 10.1002/ar.1128
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Fetched: 2026-07-19 MST

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APA

Young, W., Steele, T.A., Bray, R.A., Hudson, J., Floyd, J.A., Hawkins, K.C., Thomas, K., Austin, T., Edwards, C., Cuzzourt, J., Duenzl, M., Lucas, P.A., & Black, A.C. (2001). Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors. <em>The Anatomical Record</em>. https://doi.org/10.1002/ar.1128

Vancouver

Young W, Steele TA, Bray RA, Hudson J, Floyd JA, Hawkins KC, et al. Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors. The Anatomical Record. 2001. doi:10.1002/ar.1128.

BibTeX

@article{warren2001Humanr, title = {Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors}, author = {Warren Young and Timothy A. Steele and Robert A. Bray and John Hudson and Julie A. Floyd and Kristina C. Hawkins and Karen Thomas and Troy Austin and Chris Edwards and Jeremy Cuzzourt and Mary Duenzl and Paul A. Lucas and Asa C. Black}, journal = {The Anatomical Record}, year = {2001}, doi = {10.1002/ar.1128}, }