Effects of Klotho, Akt and Foxo3a mRNA and protein expression in hippocampus of rats with syndrome of yang deficiency of spleen and kidney

Objective: To investigate the mechanism which of the kidney and spleen-yang deficiency responsible for aging, as well as impact on klotho, Akt, Foxo3 a mRNA and protein expression of Guifu Lizhong Pill. Methods: Model rats with syndrome of yang deficiency of spleen and kidney were established. Real-time fluorescent quantitative PCR, Western Blot and immunohistochemistry were used to test the mRNA and protein expression of Klotho, Akt and Foxo3 a. Results: The expression of Klotho protein was decreased in hippocampus of rats with syndrome of yang deficiency of spleen and kidney, the phosphorylation reaction of Akt and Foxo3 a in downstream of signal transduction pathway was enhanced, and the expression of Akt and Foxo3 a protein was increased. Guifu Lizhong pill could enhance the expression of Klotho mRNA and protein, the inhibition of Akt, Foxo3 a phosphorylation, thereby increased SOD2(MnSOD) expression. Conclusion: Down-regulated expression of Klotho may be the mechanism of aging of rats with syndrome of yang deficiency of spleen and kidney, and this research provids molecular basis for the pathomechanism of inducing aging by yang deficiency of spleen and kidney.