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Age related changes in cell stiffness of tendon stem/progenitor cells and a rejuvenating effect of ROCK-inhibition

Stefanie Kiderlen, Christoph Polzer, Joachim Oskar Rädler, Denitsa Docheva, Hauke Clausen‐Schaumann, Stefanie Sudhop

Biochemical and Biophysical Research Communications · 2019 · ▲ 32 citations

Stem-cell exhaustion Partial reprogramming (OSK) Human

Abstract

Tendon stem/progenitor cells (TSPC) are potential targets for regenerative medicine and the treatment of tendon injuries. The frequency of such injuries increases in elderly patients while the proportion of functional TSPCs in tendon tissue decreases, protracting tendon repair. Using atomic force microscopy (AFM), we show that cell stiffness and size increase in TSPCs isolated from elderly patients (A-TSPC) compared to TSPCs from younger patients (Y-TSPC). Additionally, two-photon excited fluorescence (TPEF) microscopy revealed a denser, well-structured actin cytoskeleton in A-TSPC, which correlates with the augmented cell stiffness. Treating A-TSPC with ROCK-inhibitor, reverses these age-related changes, and has rejuvenating effect on cell morphology and stiffness. We assume that cellular stiffness is a suitable marker for cell aging and ROCK a potential target for therapeutic applications of cell rejuvenation.

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Provenance

Source: OpenAlex
DOI: 10.1016/j.bbrc.2019.01.027
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Fetched: 2026-10-24 MST

Cite this

APA

Kiderlen, S., Polzer, C., Rädler, J.O., Docheva, D., Clausen‐Schaumann, H., & Sudhop, S. (2019). Age related changes in cell stiffness of tendon stem/progenitor cells and a rejuvenating effect of ROCK-inhibition. <em>Biochemical and Biophysical Research Communications</em>. https://doi.org/10.1016/j.bbrc.2019.01.027

Vancouver

Kiderlen S, Polzer C, Rädler JO, Docheva D, Clausen‐Schaumann H, Sudhop S. Age related changes in cell stiffness of tendon stem/progenitor cells and a rejuvenating effect of ROCK-inhibition. Biochemical and Biophysical Research Communications. 2019. doi:10.1016/j.bbrc.2019.01.027.

BibTeX

@article{stefanie2019Agerel, title = {Age related changes in cell stiffness of tendon stem/progenitor cells and a rejuvenating effect of ROCK-inhibition}, author = {Stefanie Kiderlen and Christoph Polzer and Joachim Oskar Rädler and Denitsa Docheva and Hauke Clausen‐Schaumann and Stefanie Sudhop}, journal = {Biochemical and Biophysical Research Communications}, year = {2019}, doi = {10.1016/j.bbrc.2019.01.027}, }