Open access · OA via Europe PMC

Multivalent binding of the tardigrade Dsup protein to chromatin promotes yeast survival and longevity upon exposure to oxidative damage.

Aguilar RR, Khan LF, Cummins CK, Arslanovic N, Grauer T, Birmingham K, Kasliwal K, Posnikoff SDL, Chakraborty U, Hickman AR, Watson R, Ezell RJ, Hunt SR, Mukhsinova L, Willis HE

Nature communications · 2025 · ▲ 9 citations

Genomic instability Epigenetic alterations Mitochondrial dysfunction Yeast

＋ Sign in to save

Abstract

Tardigrades are remarkable in their ability to survive extreme environments. The damage suppressor (Dsup) protein is thought to contribute to their extreme resistance to reactive oxygen species (ROS) generated by irradiation. Here we show that expression of Ramazzottius varieornatus Dsup in Saccharomyces cerevisiae reduces oxidative DNA damage and extends lifespan in response to chronic oxidative genotoxicity. Dsup uses multiple modes of engagement with the nucleosomal H2A/H2B acidic patch, H3/H4 histone tails and DNA to bind across the yeast genome without bias. Effective chromatin binding and genome protection requires the Dsup HMGN-like motif and C-terminal sequences. These findings give precedent and mechanistic understanding for engineering an organism by physically shielding its genome to promote survival and longevity in the face of oxidative damage.

◌ CITATION ONLY
Full text is not openly licensed for redistribution here. Read it at the source:

Read at source →

Provenance

Source: Europe PMC
DOI: 10.1038/s41467-025-63652-3
Canonical: link ↗
Fetched: 2026-07-01 MST

Cite this

APA

RR, A., LF, K., CK, C., N, A., T, G., K, B., K, K., SDL, P., U, C., AR, H., R, W., RJ, E., SR, H., L, M., HE, W., MW, C., R, G., A, S., JI, G., & BJ, V. (2025). Multivalent binding of the tardigrade Dsup protein to chromatin promotes yeast survival and longevity upon exposure to oxidative damage. <em>Nature communications</em>. https://doi.org/10.1038/s41467-025-63652-3

Vancouver

RR A, LF K, CK C, N A, T G, K B, et al. Multivalent binding of the tardigrade Dsup protein to chromatin promotes yeast survival and longevity upon exposure to oxidative damage. Nature communications. 2025. doi:10.1038/s41467-025-63652-3.

BibTeX

@article{aguilar2025Multiv, title = {Multivalent binding of the tardigrade Dsup protein to chromatin promotes yeast survival and longevity upon exposure to oxidative damage.}, author = {Aguilar RR and Khan LF and Cummins CK and Arslanovic N and Grauer T and Birmingham K and Kasliwal K and Posnikoff SDL and Chakraborty U and Hickman AR and Watson R and Ezell RJ and Hunt SR and Mukhsinova L and Willis HE and Cowles MW and Garner R and Shim A and Gutierrez JI and Venters BJ and Marunde MR and Strahl BD and Keogh MC and Tyler JK.}, journal = {Nature communications}, year = {2025}, doi = {10.1038/s41467-025-63652-3}, }